Chemical Composition of Pinus brutia Ten Essential Oil and Its in vitro Anti-Inflammatory Activity
Lina Karrat *
Department of Pharmacognosy, Faculty of Pharmacy, University of Aleppo, Aleppo, Syria.
Ream Nayal
Department of Pharmacognosy, Faculty of Pharmacy, University of Aleppo, Aleppo, Syria.
Mohammad Yaser Abajy
Department of Biochemistry and Microbiology, Faculty of Pharmacy, University of Aleppo, Aleppo, Syria.
*Author to whom correspondence should be addressed.
Abstract
This investigation aims to determine the chemical composition of Pinus brutia leaves essential oil and evaluate its anti-inflammatory property using Human Red Blood Cells (HRBC) membrane stabilization assay and Albumin denaturation assay. The chemical composition of essential oil (EO) obtained by hydro-distillation of leaves of Pinus brutia was investigated by GC-MS. The anti-inflammatory effect of EO was evaluated using Human Red Blood Cells (HRBC) membrane stabilization assay and Albumin denaturation assay. The main constituents of EO were α-Terpineol (66.16%), 3-Carene (4.90%), Carveol (4.55%) and cis-Verbenol (3.22%). The inhibion of hemolysis was observed at concentrations (2.5-12.5) µg/ml. Moreover, albumin denaturation test showed protection effect at concentrations (8-40) µg/ml. We concluded that, Pinusbrutia EO shows strong anti-inflammatory activity at different concentration when compared to standard drug of Diclofenac sodium. In addition, GC-MS analysis of Pinus brutia EO showed the presence of α-Terpineol as major compound in the oil. It reveals that this constituent is responsible to maximum protection of albumin denaturation and membrane stabilization assay. The future work will be determination of anti-inflammatory by in vivo models.
Keywords: Albumin denaturation assay, essential oil, HRBC test, GC-MS, Pinus brutia.